Journal: The Cochrane Database of Systematic Reviews

Article Title: Diet, physical activity and behavioural interventions for the treatment of overweight or obese children from the age of 6 to 11 years

doi: 10.1002/14651858.CD012651

Figure Lengend Snippet:

Article Snippet: Methods Parallel RCT Randomisation ratio : 1:1 Superiority design Participants Inclusion criteria : aged 10‐14 years, overweight or obese (IOTF cut offs), owned a Playstation 2 or 3 gaming console (Sony Computer Entertainment Inc, Tokyo, Japan), but no active video games (including EyeToy (Sony) or NintendoWii, played ≥ 2 h of video games per week, only 1 child per household was eligible to take part in the study Exclusion criteria : contraindications to performing physical activity (e.g. medical conditions) Diagnostic criteria : see above Interventions Number of study centres : 1 Run‐in period : no Extension period : no Intervention: active video game package Comparator: no‐care control group Outcomes Outcome measures reported in abstract : BMI, percentage body fat, daily time spent playing active video games and nonactive video games Study details Trial terminated early: no Trial ID: ACTRN12607000632493 Publication details Language of publication : English Funding: Health Research Council of New Zealand (grant 07/077B), a Heart Foundation of New Zealand Fellowship (RM), a Heart Foundation of New Zealand Senior Fellowship (CNM), and a Tertiary Education Commission Bright Futures Doctoral Scholarship (LF).

Techniques: Control, Standard Deviation

Journal: The Cochrane Database of Systematic Reviews

Article Title: Diet, physical activity and behavioural interventions for the treatment of overweight or obese children from the age of 6 to 11 years

doi: 10.1002/14651858.CD012651

Figure Lengend Snippet: Berry 2007

Article Snippet: Methods Parallel RCT Randomisation ratio : 1:1 Superiority design Participants Inclusion criteria : aged 10‐14 years, overweight or obese (IOTF cut offs), owned a Playstation 2 or 3 gaming console (Sony Computer Entertainment Inc, Tokyo, Japan), but no active video games (including EyeToy (Sony) or NintendoWii, played ≥ 2 h of video games per week, only 1 child per household was eligible to take part in the study Exclusion criteria : contraindications to performing physical activity (e.g. medical conditions) Diagnostic criteria : see above Interventions Number of study centres : 1 Run‐in period : no Extension period : no Intervention: active video game package Comparator: no‐care control group Outcomes Outcome measures reported in abstract : BMI, percentage body fat, daily time spent playing active video games and nonactive video games Study details Trial terminated early: no Trial ID: ACTRN12607000632493 Publication details Language of publication : English Funding: Health Research Council of New Zealand (grant 07/077B), a Heart Foundation of New Zealand Fellowship (RM), a Heart Foundation of New Zealand Senior Fellowship (CNM), and a Tertiary Education Commission Bright Futures Doctoral Scholarship (LF).

Techniques: Biomarker Discovery, Diagnostic Assay, Sequencing, Selection, Control

Journal: The Cochrane Database of Systematic Reviews

Article Title: Diet, physical activity and behavioural interventions for the treatment of overweight or obese children from the age of 6 to 11 years

doi: 10.1002/14651858.CD012651

Figure Lengend Snippet: Berry 2014

Article Snippet: Methods Parallel RCT Randomisation ratio : 1:1 Superiority design Participants Inclusion criteria : aged 10‐14 years, overweight or obese (IOTF cut offs), owned a Playstation 2 or 3 gaming console (Sony Computer Entertainment Inc, Tokyo, Japan), but no active video games (including EyeToy (Sony) or NintendoWii, played ≥ 2 h of video games per week, only 1 child per household was eligible to take part in the study Exclusion criteria : contraindications to performing physical activity (e.g. medical conditions) Diagnostic criteria : see above Interventions Number of study centres : 1 Run‐in period : no Extension period : no Intervention: active video game package Comparator: no‐care control group Outcomes Outcome measures reported in abstract : BMI, percentage body fat, daily time spent playing active video games and nonactive video games Study details Trial terminated early: no Trial ID: ACTRN12607000632493 Publication details Language of publication : English Funding: Health Research Council of New Zealand (grant 07/077B), a Heart Foundation of New Zealand Fellowship (RM), a Heart Foundation of New Zealand Senior Fellowship (CNM), and a Tertiary Education Commission Bright Futures Doctoral Scholarship (LF).

Techniques: Diagnostic Assay, Control, Sequencing, Selection

Journal: The Cochrane Database of Systematic Reviews

Article Title: Diet, physical activity and behavioural interventions for the treatment of overweight or obese children from the age of 6 to 11 years

doi: 10.1002/14651858.CD012651

Figure Lengend Snippet: ACTRN12613001037796

Article Snippet: Methods Parallel RCT Randomisation ratio : 1:1 Superiority design Participants Inclusion criteria : aged 10‐14 years, overweight or obese (IOTF cut offs), owned a Playstation 2 or 3 gaming console (Sony Computer Entertainment Inc, Tokyo, Japan), but no active video games (including EyeToy (Sony) or NintendoWii, played ≥ 2 h of video games per week, only 1 child per household was eligible to take part in the study Exclusion criteria : contraindications to performing physical activity (e.g. medical conditions) Diagnostic criteria : see above Interventions Number of study centres : 1 Run‐in period : no Extension period : no Intervention: active video game package Comparator: no‐care control group Outcomes Outcome measures reported in abstract : BMI, percentage body fat, daily time spent playing active video games and nonactive video games Study details Trial terminated early: no Trial ID: ACTRN12607000632493 Publication details Language of publication : English Funding: Health Research Council of New Zealand (grant 07/077B), a Heart Foundation of New Zealand Fellowship (RM), a Heart Foundation of New Zealand Senior Fellowship (CNM), and a Tertiary Education Commission Bright Futures Doctoral Scholarship (LF).

Techniques: Control, Medications, Biomarker Discovery, Activity Assay

Journal: Stem Cells Translational Medicine

Article Title: Enhanced Expansion and Sustained Inductive Function of Skin‐Derived Precursor Cells in Computer‐Controlled Stirred Suspension Bioreactors

doi: 10.5966/sctm.2016-0133

Figure Lengend Snippet: Experimental timeline for cell inoculation, serial passaging in bioreactor and static conditions, and patch assay and homing assay transplantations. Abbreviations: d, day; NOD/SCID, nonobese diabetic/severe combined immunodeficiency; SKP, skin‐derived precursor.

Article Snippet: The overall experimental design is shown in . rSKPs from each animal were cultured for three consecutive passages in 500‐ml computer controlled DASGIP Parallel Bioreactor Systems (Eppendorf, Hamburg, Germany, http://www.eppendorf.com ) and, in parallel, in T75 suspension culture flasks (Cellstar; Greiner Bio One, Kremsmünster, Austria, http://www.gbo.com ).

Techniques: Passaging, Derivative Assay

Journal: Stem Cells Translational Medicine

Article Title: Enhanced Expansion and Sustained Inductive Function of Skin‐Derived Precursor Cells in Computer‐Controlled Stirred Suspension Bioreactors

doi: 10.5966/sctm.2016-0133

Figure Lengend Snippet: Rat skin‐derived precursor (rSKP) cells grown in computer‐controlled bioreactors produce significantly greater numbers of viable cells over multiple passages. (A): Representative images showing rSKPs continue to grow as aggregates over three consecutive passages in static and bioreactor conditions. (B): Multiplication ratios over serial passages in static and bioreactor conditions. A significant difference was found in the multiplication ratios between passages 1 and 2 in bioreactor and static conditions ( p < .0001) and between passages 2 and 3 in the bioreactor condition ( p < .05). By the third serial passage, a significant difference was found in the cumulative multiplication ratios between the bioreactor and static conditions ( p < .05). (C): Cumulative multiplication ratios over serial passages in static and bioreactor conditions. At the end of each serial passage, the average aggregate size in the bioreactor condition was significantly smaller than in the static condition ( p < .0001). (D): Average aggregate size of rSKP cells in static and bioreactor conditions. (E): Exponential doubling times in static and bioreactor conditions. (F, G): The bioreactor condition was under continuous feedback control regulating the dissolved oxygen concentration in the medium at 21% and the pH at 7.4. Scale bars = 200 μm. A two‐way analysis of variance (ANOVA) followed by Sidak's multiple comparison test was used for (B–D) , and a one‐way ANOVA followed by Sidak's multiple comparison test was used for (E) . Error bars represent SEM of three independent experiments. ****, p < .0001; ***, p < .001; *, p < .05.

Article Snippet: The overall experimental design is shown in . rSKPs from each animal were cultured for three consecutive passages in 500‐ml computer controlled DASGIP Parallel Bioreactor Systems (Eppendorf, Hamburg, Germany, http://www.eppendorf.com ) and, in parallel, in T75 suspension culture flasks (Cellstar; Greiner Bio One, Kremsmünster, Austria, http://www.gbo.com ).

Techniques: Derivative Assay, Control, Concentration Assay, Comparison

Journal: Stem Cells Translational Medicine

Article Title: Enhanced Expansion and Sustained Inductive Function of Skin‐Derived Precursor Cells in Computer‐Controlled Stirred Suspension Bioreactors

doi: 10.5966/sctm.2016-0133

Figure Lengend Snippet: Characteristic skin‐derived precursor (SKP) markers in bioreactor versus static culture. (A–K): Flow cytometry of single‐cell rat SKPs grown for three consecutive passages under static and bioreactor conditions was performed to assess the protein expression of characteristic SKP markers. (L): No significant difference was found in the percentage of positive cell expression between the static and bioreactor cultures. Two‐tailed t test, p = nonsignificant. Error bars represent SEM of three independent experiments. Abbreviations: APC‐A, allophycocyanin; FB, fibronectin; FSC, forward scatter; FSP1, fibroblast‐specific protein 1; PDGFR‐α, platelet‐derived growth factor receptor‐α; PE‐A, r‐phycoerythrin; α‐SMA, α‐smooth muscle actin.

Article Snippet: The overall experimental design is shown in . rSKPs from each animal were cultured for three consecutive passages in 500‐ml computer controlled DASGIP Parallel Bioreactor Systems (Eppendorf, Hamburg, Germany, http://www.eppendorf.com ) and, in parallel, in T75 suspension culture flasks (Cellstar; Greiner Bio One, Kremsmünster, Austria, http://www.gbo.com ).

Techniques: Derivative Assay, Flow Cytometry, Expressing, Two Tailed Test

Journal: Stem Cells Translational Medicine

Article Title: Enhanced Expansion and Sustained Inductive Function of Skin‐Derived Precursor Cells in Computer‐Controlled Stirred Suspension Bioreactors

doi: 10.5966/sctm.2016-0133

Figure Lengend Snippet: rSKPs repopulate the hair follicle (HF) mesenchyme after either bioreactor or static culture. (A): Representative images of endogenous HFs containing transplanted GFP + rSKPs (green) from static and bioreactor grown rSKPs. Skin sections were immunostained for versican (red). (B): Percentage of GFP + HFs. (C): Percentage of GFP + dermal papilla HFs between static and bioreactor culture conditions. Scale bars = 100 μm. Two‐tailed t test, p = ns. Error bars represent SEM of three independent experiments. Abbreviations: DP, dermal papilla; GFP + , green fluorescent protein positive; ns, not significant; rSKP, rat skin‐derived precursor.

Article Snippet: The overall experimental design is shown in . rSKPs from each animal were cultured for three consecutive passages in 500‐ml computer controlled DASGIP Parallel Bioreactor Systems (Eppendorf, Hamburg, Germany, http://www.eppendorf.com ) and, in parallel, in T75 suspension culture flasks (Cellstar; Greiner Bio One, Kremsmünster, Austria, http://www.gbo.com ).

Techniques: Two Tailed Test, Derivative Assay

Journal: Stem Cells Translational Medicine

Article Title: Enhanced Expansion and Sustained Inductive Function of Skin‐Derived Precursor Cells in Computer‐Controlled Stirred Suspension Bioreactors

doi: 10.5966/sctm.2016-0133

Figure Lengend Snippet: GFP rat skin‐derived precursors (rSKPs) from bioreactor and static culture retain their capacity to induce hair follicle (HF) morphogenesis. (A): Representative low‐magnification images of grafts containing bioreactor or static cultured GFP + rSKPs combined with keratinocytes after 14 days. (B): Individually dissected HFs showing integration of GFP + cells in the dermal papilla (DP) and the connective tissue sheath. (C): Number of GFP + HFs formed within grafts containing either static‐ or bioreactor‐generated SKPs. Two‐tailed t test, p = .023. (D): Quantification of HF formation in the patch assay, with inclusion of wild‐type dermal fibroblasts to improve survival of donor cells. Note, despite supplementing each graft, bioreactor‐expanded GFP + cells showed a sustained impairment. Error bars represent SEM of three independent experiments. Image magnifications are ×5 (A) and ×20 (B) . Abbreviation: GFP, green fluorescent protein.

Article Snippet: The overall experimental design is shown in . rSKPs from each animal were cultured for three consecutive passages in 500‐ml computer controlled DASGIP Parallel Bioreactor Systems (Eppendorf, Hamburg, Germany, http://www.eppendorf.com ) and, in parallel, in T75 suspension culture flasks (Cellstar; Greiner Bio One, Kremsmünster, Austria, http://www.gbo.com ).

Techniques: Derivative Assay, Cell Culture, Generated, Two Tailed Test

Journal: The Journal of Biological Chemistry

Article Title: Binding of the Human Complement Regulators CFHR1 and Factor H by Streptococcal Collagen-like Protein 1 (Scl1) via Their Conserved C Termini Allows Control of the Complement Cascade at Multiple Levels *

doi: 10.1074/jbc.M110.143727

Figure Lengend Snippet: CFHR1 bound to Scl1.6 and Scl1.55 inhibits TCC formation. A, the functional relevance of CFHR1·Scl1.6 and CFHR1·Scl1.55 complexes was analyzed in a hemolysis assay with sheep erythrocytes. TCC formation on sheep erythrocytes was induced with C5b6, C7, C8, and C9 and assayed by following erythrocyte lysis. Lysis was inhibited by increasing amounts of CFHR1·Scl1.6 and CFHR1·Scl1.55 complexes. Scl1.6 alone had a minor effect on hemolysis that was not amplified by increasing amounts. The mean values ± S.D. of three independent experiments are shown. B, TCC deposition was analyzed by the addition of TCC compounds C5b6, C7, C8, and C9 to CFHR1 bound to immobilized Scl1.6 and Scl1.55. For the negative control sample (no TCC), no C9 was added. TCC was detected using a TCC-specific antiserum. CFHR1 bound to Scl1.6 and Scl1.55 significantly reduced TCC deposition, whereas bound Factor H did not affect the TCC. Scl2.28, which bound to neither CFHR1 nor Factor H, did not influence TCC deposition. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: In parallel, purified TCC compounds (Comptech) diluted in HEPES buffer (20 m m HEPES, 144 m m NaCl, 7 m m MgCl 2 , and 10 m m EGTA) were added to the wells.

Techniques: Functional Assay, Hemolysis Assay, Lysis, Amplification, Negative Control